FVSUag Directory

singhm

Mahipal Singh, PhD, CRA

Professor of Animal Biotechnology, Animal Science Undergraduate Program Coordinator

(478) 825-7042

(478) 825-6376

singhm@fvsu.edu

Academic Program: Animal Science

Research Area: Small Ruminants

Research Interests:
  • Animal biotechnology
  • Molecular biology
  • In‐vitro cell culture
  • Genome‐editing
  • Stem cells
  • Livestock reproduction
Several images depicting activities in Dr. Singh's animal biotechnology lab (from top left): A). outgrowth of cells from tissues. B). recovery of live cells up to 41 days after animal death. C). growth profile of recovered cells. D). GFP gene expression in recovered cells. E). normal karyotype. F &G). DAPI and vimentin staining. H&I). mammary epithelial cells. J). restriction map of pPRO-3 plasmid. K). in vitro fertilization in livestock. L). in vitro produced embryo. M). a fibroblast cell line established in the lab. N&O). giPSCs developed. P). animal biotechnology team in the lab at FVSU.
Several images depicting activities in Dr. Singh's animal biotechnology lab (from top left): A). outgrowth of cells from tissues. B). recovery of live cells up to 41 days after animal death. C). growth profile of recovered cells. D). GFP gene expression in recovered cells. E). normal karyotype. F&G). DAPI and vimentin staining. H&I). mammary epithelial cells. J). restriction map of pPRO-3 plasmid. K). in vitro fertilization in livestock. L). in vitro produced embryo. M). a fibroblast cell line established in the lab. N&O). giPSCs developed. P). animal biotechnology team in the lab at FVSU.
Honors/Awards:
  • USDA‐ARS 1890 Faculty Research Fellow Award (2017‐18)
  • Provost Fellow Award, Fort Valley State University (2018‐19)
  • STEM Research Excellence Award, Fort Valley State University (2014‐15)
  • First Prize for Research Presentation, FVSU Annual Research Day symposium (2017)
  • Second Prize for Graduate student Oral Research Presentation, FVSU Annual Research Day symposium (2017)
  • Certificates of recognition for excellence in scholarly research & grantsmanship by FVSU president (2008, 2009, 2010, 2011, 2012, 2016)
  • Best Poster Award, National Symposium on Biotechnology, CIMAP, Lucknow, India (1999)
Education:

Doctor of Philosophy: Zoology-Microbiology - Banaras Hindu University, Varanasi, India (1989)

Master of Science: Zoology - Institute of Advanced Studies, Meerut University Meerut, India (1983)

Bachelor of Science (Honors): Zoology - Meerut College Meerut, Meerut University, India (1978)

Recent Publications/Presentations:

Peer‐reviewed journal articles:

Singh, M; Ben Hortman, V N Degala and X. Ma. Establishment and Characterization of a Lactating Caprine Mammary Gland Luminal Epithelial Cell Line. International Journal of Biology (2019) 11(3): 35‐41. (DOI:10.5539/ijb.v11n4p36 )

Singh, M and X. Ma. Nucleofection Efficiency of Sheep Primary Fibroblasts Established from Refrigerated Skin. International Journal of Biology. (2018) 10(4): 12‐15. (DOI:10.5539/ijb.v10n4p12)

Brian, W.; and M. Singh. Recovery of proliferative cells up to 15‐ and 49‐days postmortem from bovine skin stored at 25°C and 4°C, respectively. Cogent Biology (2017) 3: 1333760 (https://doi.org/10.1080/23312025.2017.1333760).

Singh, M; and N Degala. Postmortem recovery of proliferative fibroblasts up to three days in livestock ear skin stored at 35°C. International Journal of Biology. (2017) 9(2): 9‐13. (https://doi.org/10.5539/ijb.v9n2p9)

Singh, M; K McKenzie, and X. Ma. Effect of dimethyl sulfoxide on in vitro proliferation of skin fibroblast cells. Journal of Biotech Research (2017) 8: 78‐82.

Aoued, HS; and M. Singh. Recovery of Fibroblast‐Like Cells after 160 Days of Postmortem Storage of Goat Skin Tissues in Refrigerated Media. Journal of Veterinary Science and Technology (2015) 6: 4 (DOI: 10.4172/2157‐7579.1000236)

Okonkwo, C; and M. Singh. Recovery of fibroblast‐like cells from refrigerated goat skin up to 41 days of animal death. In Vitro Cell and Developmental Biology‐Animal. (2015) 51: 463‐69 (DOI: 10.1007/s11626‐014‐9856‐9)

Singh, M; and X. Ma. In Vitro Culture of Fibroblast‐Like Cells From Sheep Ear Skin Stored at 25‐26°C for 10 Days After Animal Death. International J. of Biology (2014) 6 (4): 96‐102 (doi: 10.5539/ijb.v6n4p96)

Mehta R; R Arya, K Goyal, M Singh and A K Sharma. Bio‐preservative and therapeutic potential of pediocin: recent trends and future perspectives. Recent Patents on Biotechnology (2013) 7(3): 172‐78 (doi: 10.2174/18722083113076660008)

Adefenwa, MA, B O Agaviezor, SO Peters, M Wheto, K Adekoya, M Okpeku, B Oboh, GO Williams, M Singh and IG Imumorin. Identification of ingle nucleotide polymorphisms in the agouti signaling protein (ASIP) gene in Nigerian and North American goats. Molecular Biology Reports (2013) 40(7): 4447‐4457 (doi: 10.1007/s11033‐013‐2535‐1)

Singh, M; X. Ma & A. Sharma. Effect of postmortem time interval on in vitro culture potential of goat skin tissues stored at room temperature. In Vitro Cell and Developmental Biology-Animal. (2012) 48: 478‐482 (doi: 10.1007/s11626‐012‐9539‐3)

Singh, M; B. Dhiman, C. Sharma. Characterization of a highly repetitive DNA sequence in Camellia sinensis (L.) O. Kuntze genome. Journal of Biotech Research (2011) 3: 78‐83

Patents:

Singh, M; C Sharma & B Lal. Species‐specific DNA sequences and their utilization for identification of Viola species and in detection of adulteration in a medicinal herb “banafsha”. US patent no: 6465637 granted in 2001.

Singh, M & B Dhiman. Species‐specific genomic DNA sequences for identification of Anacardium occidentale and the method for their utilization in detection of adulteration of cashew husk in tea samples. US patent no: 6541624 granted in 2001.

Book chapters:

Ahmad, I; MSA Khan, F Aqil & M Singh*. Microbial applications in agriculture and the environment: a broad perspective. In: Iqbal Ahmad, F. Ahmad & J. Pichtel. Eds. Microbes and Microbial Technology: Agriculture and Environmental Applications; Springer New York (2011) pp.1‐27. (doi: 10.1007/978‐1‐4419‐7931‐5_1) (*Corresponding author).

Ahmad, I; MSA Khan, FM Husain, M Zahin & M Singh. Bacterial Quorum Sensing and Its Interference: Methods and Significance. In: Iqbal Ahmad, A. Ahmad and J. Pichtel. Eds. Microbes and Microbial Technology: Agricultural and Environmental Applications; Springer New York (2011) pp.127‐161. (doi: 10.1007/978‐1‐4419‐7931‐5_6)

DNA sequences published in public database GENBANK:

Adefenwa, M A; B O Agaviezor, S O Peters, M Wheto, K O Adekoya, Okpeku,M., Oboh,B., Williams,G.O., Singh, M., De Donato,M., Thomas,B. and Imumorin,I.G. Capra hircus clone 94 melanocortin 1 receptor (MC1R) gene (& association with coat color), partial cds. GenBank Accession Number: KC476442.

Singh, M; E Amoah; G Kannan, SL Stice & DM Donovan. Capra hircus myostatin upstream region genomic sequence. Accession number: GQ121039.

Singh, D & M Singh. Structure of 5S ribosomal RNA gene repeat units in Camellia sinensis. Accession number: AY101390

Singh, M; B Dhiman, C Sharma; D Singh (2002): Cloning, sequencing and characterization of a highly repetitive DNA fragment from Camellia sinensis L. (O.) Kuntze genome. [Include (AG)n microsatellites]. Accession number: AF546881

Singh, M & AK Yadav. Organization of 5S ribosomal RNA gene in Carica quercifolia. Accession number: AF506758

Singh, M; C Sharma & B Lal. Viola species‐specific DNA sequences. Accession numbers: AR237797, AR237798, AR237799, AR237800, AR237801, AR237802, AR237803, AR237804, AR237805, AR237806, AR237807 and AR237808 (parts of Singh patent)

Singh, M & B Dhiman. 5S ribosomal RNA intergenic spacer regions of detective value isolated from Anacardium occidentale, Camellia sinensis, and Dendrocalamus hamiltonii. Accession numbers: AY230649, AY230650 and AY230651, respectively.

Abstracts published (in conferences/professional society meeting proceedings):

Singh, M; Ki‐Eun Park, W Tyler, G McCommon, T Bhanu, M Knight, A Gillespie, X Ma, A Powel, D Donovan, T Ramsay, PSXIII‐40 Superovulation and oocyte recovery rates in CIDR synchronized goats treated with PGF2α, FSH and GnRH during breeding season, Journal of Animal Science, Volume 97, Issue Supplement 3, December 2019, Page 367 (https://doi.org/10.1093/jas/skz258.732)

Singh, M; B Hortman, V N. Degala and X Ma. Establishment and Preliminary Characterization of a Lactating Caprine Mammary Gland Luminal Epithelial Cell Line. Presented in the Society for In Vitro Biology Meeting, Tempa, FL, June 8‐12, 2019.

Weekes, K; T Williams, X Ma, and M Singh. Transfection of Fibroblast cells by plasmid DNA using 4D Nucleofection. FVSU 9th Annual Research Day, April 18, 2019. Abstract on page 12.

#,*K. Weekes*, T. Williams, X. Ma, and M. Singh. (2019). Efficient Transfection of Goat Primary Fibroblasts by plasmid DNA using 4D Nucleofection System. Presented by undergraduate student at National Council of Undergraduate Research (NCUR) Conference at the Kennesaw State University, April 10‐13, 2019. (https://apps.cur.org/ncur2019/search/Display_NCUR.aspx?id=114214)

#,*A. Kutagulla*, X. Ma, and M. Singh, Recovery of Proliferative Cells from Refrigerated Visceral Organs in Livestock. Presented in 19th Biennial ARD Research symposium, Jacksonville, FL, March 30, 2019.

#,*K. Weeks, T. Williams*, X. Ma, and M. Singh, Optimization of Plasmid DNA Concentration for Efficient Transfection of Goat Primary Fibroblasts using Nucleofection. Presented in 19th Biennial ARD Research symposium, Jacksonville, FL, March 30, 2019.

Singh, M, Degala V. PSVI‐36 Recovery of progenitor cells from skin tissues stored at elevated temperatures postmortem. , Journal of Animal Science, 2018 Dec; 96 (Suppl 3): 465–466. (doi: 10.1093/jas/sky404.1017). Epub 2018 Dec 7. PMCID: PMC6285161.

#,*Neal, A.; X Ma and M. Singh. Cryopreservation of goat ovarian tissues for fertility restoration. Presented by my undergraduate student in FVSU 8th Annual Research Day, April 12, 2018. Abstract on page 13.

$, #,*Renee Bynum; X Ma and M. Singh. Comparison of the cryopreservation rates of goat tissues using a commercial kit and a lab made kit. Presented by my undergraduate student in 33rd MANRRS Annual Conference, held in Greensboro NC, April 4‐7, 2018. (First prize winner out of 13 contestants)

#,*Neal, A.; X Ma and M. Singh. Cryopreservation of ovarian tissues for fertility restoration in goats. Presented by my undergraduate student in 33rd MANRRS Annual Conference, held in Greensboro NC, April 4‐7, 2018.

#Arora, A and M. Singh. Optimizing goat skin fibroblast culture conditions for nuclear cloning. Presented in Biomedical Section of Annual Meeting of the Georgia Academy of Science, held at the University of West Georgia in Carrollton, GA on April 13‐14th, 2018. Georgia Journal of Science Vol 76: (1) article 48. (https://digitalcommons.gaacademy.org/gjs/vol76/iss1/48)

#,*McKenzie, K; X. Ma and M. Singh. Effect of Dimethyl Sulfoxide (DMSO) on In Vitro Proliferation of Mammalian Cells. Presented by my undergraduate student in 18th Biennial ARD Research symposium, Atlanta, GA April 2‐5, 2017.

$, #,*B. Hortman, M. Singh, X. Ma, and N. Degala Establishment of Caprine Mammary Luminal Epithelial Cell Lines. FVSU 7th Annual Research Day, April 20, 2017. Abstract No: 23, pp 19. (Third Prizewinner).

$, #,*McKinzie, K; and M. Singh. Effect of Dimethyl Sulfoxide (DMSO) on goat skin fibroblast cells in In Vitro culture assays. Presented by my undergraduate student in FVSU 7th Annual Research Day, April 20, 2017. Abstract No: 6, pp 13. (First Prize winner)

*Singh, M and B Walcott. In vitro culture of cells up to 15‐ and 49‐days postmortem in bovine skin stored at 25°C and 4°C, respectively. Presented in American Society of Cell Biology meeting, San Francisco, CA, December 3‐7, 2016; Poster no: P879 (Board no: B252)

*Singh, M and X. Ma. Recovery of fibroblast cells up to 65 days of postmortem storage of sheep ear skin at 4°C. Presented in American Society of Animal Science meeting, Salt Lake City, July 17‐ 19, 2016.

$,#Spratling, W; S. A. Aoued, O. J. Ojo, A.I. Younis, E. Amoah and M. Singh. Comparison of oocyte recovery from slaughtered animal ovaries in three livestock species and their quality assessment. FVSU 6th Annual Research Day, April 14, 2016. Abstract No: 16, pp 14‐15. (2nd Prize winner)

Singh, M* and B Walcott. Isolation of Fibroblast‐like Cells after 42 Days of Postmortem Storage of Cattle Skin in a Refrigerator. In Vitro Cell. Dev. Biol.‐Animal (2016) 52 (Suppl.1): page 6 (Abstr.). Poster no: A‐3000. Presented in the World Forum on In Vitro Biology, Davis CA, June 11‐ 15, 2016.

#,*Aoued, H.S., M. Singh. Recovery of fibroblast‐like cells after 160 days of postmortem storage of goat skin tissues in refrigerated media. In Vitro Cell. Dev. Biol.‐Animal (2014) 50: S40. DOI 10.1007/s11626‐014‐9765‐y (Abstr.). Interactive poster presentation no: A2002.

*Singh, M; X. Ma, G. Kannan, and E. Amoah. Time limits of postmortem cell survival in goat ear skin stored at room temperature. J. Animal Sci. (2013) 91 (E. Suppl.): pp220 (Abstr).

Singh, M, X. Ma, C. Okonkwo, N. Degala, P. Yadav, O. White, E. Amoah, W. Getz, and C. Borne. Enhancing student learning with hands‐on laboratory exercises and research projects in animal biotechnology. NACTA Journal, Vol. 57, supplement 1 (2013) pp 96‐97, abstract no 132. Presented in NACTA Conference, at Virginia Institute of Technology, June 25‐29, 2013.

Singh, M; and X. Ma. Post‐mortem Recovery of Fibroblast‐like Cells in Sheep after 10 Days of Animal Death. In Vitro Cell. Dev. Biol.‐Animal (2013) 49: S40‐46. DOI: 10.1007/S11626‐013‐ 9605‐5. (Abstr.). Interactive poster presentation no: A2000.

*Singh, M; and X. Ma. Sheep Skin Fibroblast Cell Cultures from Room Temperature Stored Tissues after Ten Days of Animal Death. 17th Biennial ARD Research symposium, Gainesville, FL April 7‐10, 2013, Abstract No: 206. Page 120.

#,*Okonkwo, C; X. Ma and M. Singh. Postmortem Cell Survival Pattern of Goat Skin Tissues Stored in Refrigerator at 4C. 17th Biennial ARD Research symposium, Gainesville, FL April 7‐10, 2013, Abstract No: 11, page 50.

#,*White, O; X. Ma, and M. Singh. Evaluation of Fetal Goat Serum as a Cheaper Alternative to Fetal Bovine Serum for Culturing Goat Cells. 17th Biennial ARD Research symposium, Gainesville, FL April 7‐10, 2013, Abstract No: 31, Page 57.

#Degala, V.N.; X. Ma, E. Amoah, and M. Singh. Development of a Cell Biological Tool for Genetic Modification of Goats. 17th Biennial ARD Research symposium, Gainesville, FL April 7‐10, 2013, Abstract No: 319, page 162.

Singh, M; X. Ma and A. K. Sharma. In vitro culture of fibroblast‐like cells from room temperature stored goat tissue. In Vitro Cell. Dev. Biol.‐Animal (2012) 48: S50‐51. (Abstr.).

*Singh, M; and X. Ma. Ability to culture of cells from postmortem goat skin tissues stored at room temperature for different time intervals. J. Animal Sci. (2012) 90 (Suppl.3): 674 (Abstr).

*Singh, M. Can dead animals be revived after several days of their death? Oral presentation given in 2nd FVSU Research Day, April 12, 2012. Abstract No: O7, p34

Invited presentations (selected):

Genome‐Editing in goats: FVSU experience. EMPIRE conference, Westin Jekyll Islands, GA, December 12, 2019.

Postmortem cellular life: applications in animal agriculture and human medicine. National Dairy Research Institute (NDRI), Karnal, India; March 16, 2015.

How long individual body cells live postmortem in mammalian systems? Institute of Himalayan Bioresource Technology (IHBT), Palampur, India, March 19, 2015.